Selective Detection of Membrane Proteins Without Antibodies
نویسندگان
چکیده
منابع مشابه
Selective detection of membrane proteins without antibodies: a mass spectrometric version of the Western blot.
A method has been developed, called the mass western experiment in analogy to the Western blot, to detect the presence of specific proteins in complex mixtures without the need for antibodies. Proteins are identified with high sensitivity and selectivity, and their abundances are compared between samples. Membrane protein extracts were labeled with custom isotope-coded affinity tag reagents and...
متن کاملDetection and characterization of monoclonal antibodies to platelet membrane proteins
We have devised a solid-phase radioimmunoassay for the detection and characterization of monoclonal antibodies directed against platelet surface antigens. Platelet membrane proteins, solubilized with 0.1% Triton X-100, were covalently coupled to cyanogen bromide (CNBr)-activated filter paper disks that were than used as the support in antibody binding assays. SDS PAGE of solubilized membrane pr...
متن کاملMembrane proteins: Aquaporins — channels without ions
Recently determined structures have shed new light on the way that aquaporins act as passive, but selective, pores for the transport of small molecules--such as water or glycerol--across membranes.
متن کاملIdentification of rat hepatocyte plasma membrane proteins using monoclonal antibodies
We have localized and identified five rat hepatocyte plasma membrane proteins using hybridoma technology in combination with morphological and biochemical methods. Three different membrane preparations were used as immunogens: isolated hepatocytes, a preparation of plasma membrane sheets that contained all three recognizable surface domains of the intact hepatocyte (sinusoidal, lateral, and bil...
متن کاملAging of lens fibers. Mapping membrane proteins with monoclonal antibodies.
Lens fiber proteins with apparent molecular weights 57,000, 70,000, 82,000, and 100,000 were components in urea insoluble, membrane-rich fractions. Monoclonal antibodies against these proteins labeled membranes by immunofluorescence microscopy of sheep lens cryosections and are thus referred to as membrane proteins MP57, MP70, MP82, and MP100. MP70 has previously been localized in fiber junctio...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Molecular & Cellular Proteomics
سال: 2002
ISSN: 1535-9476
DOI: 10.1074/mcp.m100027-mcp200